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pet his6 sumo tev lic cloning vector  (Addgene inc)


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    Structured Review

    Addgene inc pet his6 sumo tev lic cloning vector
    Pet His6 Sumo Tev Lic Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+his6+sumo+tev/pET+His6+TEV+LIC+cloning+vector+(1B)+(Plasmid+%2329653)/bio_rxiv__64898__2026__03__29__714480-173-11-15
    Average 94 stars, based on 119 article reviews
    pet his6 sumo tev lic cloning vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Produced:

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A.
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Ligation:

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A.
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Cloning:

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A.
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Plasmid Preparation:

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A.
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Article Title: Structural basis for centromere maintenance by Drosophila CENP ‐A chaperone CAL 1
    Article Snippet: .. Primers used are shown in Table . pET3a CENP‐A 101–225 was generated in (Roure et al , ). pET His6 Sumo TEV (14S Addgene plasmid # 48291) was a gift from Scott Gradia. pEC‐K‐3C‐His was a gift from Elena Conti. pET22b H4 was a kind gift from Karolin Luger. ..

    Article Title: Molecular basis for Cdk1‐regulated timing of Mis18 complex assembly and CENP‐A deposition
    Article Snippet: Human Mis18α, Mis18α MeDiY , Mis18α C‐term , Mis18β, Mis18β MeDiY , and Mis18β C‐term were amplified from codon‐optimized sequences (GeneArt) while Mis18BP1 20–130 was amplified from a human cDNA library (MegaMan human transcriptome library, Agilent). .. Amplifications were then cloned into pET His6 msfGFP TEV, pET His6 TEV, pET His6 Sumo TEV, pET His6 MBP TEV, pGEX‐6P‐1 (GE Healthcare), pEC‐K‐3C‐His‐GST, and pEC‐K‐3C‐His LIC vectors. pET His6 msfGFP TEV (9GFP Addgene plasmid # 48287), pET His6 TEV (9B Addgene plasmid # 48284), pET His6 Sumo TEV (14S Addgene plasmid # 48291), and pET His6 MBP TEV (9C Addgene Plasmid #48286) were a gift from Scott Gradia. .. Mis18α MeDiY dimer‐disrupting mutant (Mis18α DimerM ; V82E/Y176D) and putative substrate‐binding pocket mutant (Mis18α PocketM ; L136A/Y152A/C167A/S169K) and Mis18BP1 mutants, Mis18BP1 20–130 T40E , Mis18BP1 20–130 S110D , Mis18BP1 20–130 T40E/S110D , and Mis18BP1 20–130 T40A/S110A were generated following the Quikchange site‐directed mutagenesis protocol (Stratagene).

    Article Title: Structural Basis for CAL1-Mediated Centromere Maintenance
    Article Snippet: .. Inserts and vectors were processed with T4 DNA polymerase according to manual instructions. pET His6 Sumo TEV (14S Addgene plasmid # 48291) was a gift from Scott Gradia. pEC-K-3C-His was a gift from Elena Conti. pET3a CENP-A 101-225 and pET22b H4 were kind gifts from Karolin Luger. ..

    Article Title: Spt6 is a maintenance factor for centromeric CENP-A
    Article Snippet: .. The Spt6199-338 fragment was produced as a gBlock (IDT) and used directly in ligation-independent cloning into pET-His6-Sumo TEV (14S Addgene plasmid # 48291), a gift from Scott Gradia. ..

    Generated:

    Article Title: Structural basis for centromere maintenance by Drosophila CENP ‐A chaperone CAL 1
    Article Snippet: .. Primers used are shown in Table . pET3a CENP‐A 101–225 was generated in (Roure et al , ). pET His6 Sumo TEV (14S Addgene plasmid # 48291) was a gift from Scott Gradia. pEC‐K‐3C‐His was a gift from Elena Conti. pET22b H4 was a kind gift from Karolin Luger. ..

    Clone Assay:

    Article Title: Molecular basis for Cdk1‐regulated timing of Mis18 complex assembly and CENP‐A deposition
    Article Snippet: Human Mis18α, Mis18α MeDiY , Mis18α C‐term , Mis18β, Mis18β MeDiY , and Mis18β C‐term were amplified from codon‐optimized sequences (GeneArt) while Mis18BP1 20–130 was amplified from a human cDNA library (MegaMan human transcriptome library, Agilent). .. Amplifications were then cloned into pET His6 msfGFP TEV, pET His6 TEV, pET His6 Sumo TEV, pET His6 MBP TEV, pGEX‐6P‐1 (GE Healthcare), pEC‐K‐3C‐His‐GST, and pEC‐K‐3C‐His LIC vectors. pET His6 msfGFP TEV (9GFP Addgene plasmid # 48287), pET His6 TEV (9B Addgene plasmid # 48284), pET His6 Sumo TEV (14S Addgene plasmid # 48291), and pET His6 MBP TEV (9C Addgene Plasmid #48286) were a gift from Scott Gradia. .. Mis18α MeDiY dimer‐disrupting mutant (Mis18α DimerM ; V82E/Y176D) and putative substrate‐binding pocket mutant (Mis18α PocketM ; L136A/Y152A/C167A/S169K) and Mis18BP1 mutants, Mis18BP1 20–130 T40E , Mis18BP1 20–130 S110D , Mis18BP1 20–130 T40E/S110D , and Mis18BP1 20–130 T40A/S110A were generated following the Quikchange site‐directed mutagenesis protocol (Stratagene).



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